PathHunter® Human FOXO3 Nuclear Translocation Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human FOXO3 Nuclear Translocation Cell-Based Assay Kit (U2OS) utilizes the U2OS FOXO3 Nuclear Translocation cell line. The stable engineered cell line is for use in studying drug candidates affecting the PI3 pathway. This cell line enables assessment of ligand (e.g. wortmannin) based pathway activation via detection of resulting FOXO3 nuclear translocation. This product uses EFC technology. The included cell line overexpresses PL-tagged FOXO3 and nuclear localized EA. Upon pathway activation, induced nuclear translocation of FOXO3 leads to production of the EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Nuclear Protein

Family:

Transcription factor

Protein Name:

FOXO3

Protein Aliases:

Forkhead in rhabdomyosarcoma-like 1

Accession Number:

NM_201559.2

UniProt Number:

O43524

Gene Name:

FOXO3

Gene ID:

2309

Gene Aliases:

AF6q21|FOXO2

Target Species:

Human

Cell Background:

U2OS