PathHunter® Human EphB1 Functional Kinase Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human EphB1 Functional Kinase Cell-Based Assay Kit (U2OS) utilizes the U2OS EphB1 Functional Assay Cell Line. This cell based assay is developed to study drug candidates (small molecules or biologics) targeting receptor tyrosine kinases (RTKs). This kit assesses RTK activation via the detection of EphB1 activation-induced SH2 domain protein recruitment. The included cell line overexpresses PK-tagged Human EphB1 and an EA-tagged SH2 domain protein. Upon ligand (e.g. ephrin B2)-induced receptor activation, SH2-EA recruitment leads to the production of an EFC signal proportional to the degree of receptor activation. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

EphB1

Protein Aliases:

ELK|EPH tyrosine kinase 2|EPH-like kinase 6|EK6|hEK6|Neuronally-expressed EPH-related tyrosine kinase|NET|Tyrosine-protein kinase receptor EPH-2

Accession Number:

NM_004441.3

UniProt Number:

P54762

Gene Name:

EPHB1

Gene ID:

2047

Gene Aliases:

Hek6

Target Species:

Human

Cell Background:

U2OS