PathHunter® Human Heregulin β1 (EGFR/ErbB3) Dimerization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human Heregulin β1 (EGFR/ErbB3) Dimerization Cell-Based Assay Kit (U2OS) utilizes the PathHunter® U2OS EGFR/ErbB3 Dimerization Cell Line. This assay enables the assessment of ligand (e.g. Heregulin β1)-induced dimerization of EGFR/ErbB3 pairs. The included cell line employs the PathHunter® Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress EGFR fused to PK and ErbB3 fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

EGFR

Protein Aliases:

erythroblastic leukemia viral (v-erb-b) oncogene homolog (avian)|erb-b2 receptor tyrosine kinase 1

Accession Number:

NM_005228.3

UniProt Number:

P00533

Gene Name:

EGFR

Gene ID:

1956

Gene Aliases:

ERBB1

Target Species:

Human

Cell Background:

U2OS