PathHunter® Human BMP-6 (BMPR1B/ACVR2B) Dimerization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human BMP-6 (BMPR1B/ACVR2B) Dimerization Cell-Based Assay Kit (U2OS) utilizes the PathHunter® U2OS BMPR1B/ACVR2B Dimerization Cell Line. This assay enables the assessment of ligand (e.g. BMP-6)-induced dimerization of BMPR1B/ACVR2B pairs. The included cell line employs the PathHunter® Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress BMPR1B fused to PK and ACVR2B fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Kinase

Family:

TKL

Sub Family:

Protein Ser/Thr

Protein Name:

ALK6

Protein Aliases:

BMPR-1B|CD antigen CDw293

Accession Number:

NM_001256793.1 

UniProt Number:

O00238

Gene Name:

BMPR1B

Gene ID:

658

Gene Aliases:

ALK6|CDw293

Target Species:

Human

Cell Background:

U2OS