PathHunter® Human Activin A (ACVR1B/BMPR2) Dimerization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human Activin A (ACVR1B/BMPR2) Dimerization Cell-Based Assay Kit (U2OS) utilizes the PathHunter® U2OS ACVR1B/BMPR2 Dimerization Cell Line. This assay enables the assessment of ligand (e.g. activin A)-induced dimerization of ACVR1B/BMPR2 pairs. The included cell line employs the PathHunter® Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress ACVR1B fused to PK and BMPR2 fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Kinase

Family:

RSTK

Sub Family:

Protein Ser/Thr

Protein Name:

ALK4

Protein Aliases:

Activin receptor type IB|ACTR-IB|Activin receptor-like kinase 4|ALK-4|Serine/threonine-protein kinase receptor R2|SKR2

Accession Number:

NM_004302.4

UniProt Number:

P36896

Gene Name:

ACVR1B

Gene ID:

91

Gene Aliases:

ALK4|SKR2|ActRIB|ACVRLK4

Target Species:

Human

Cell Background:

U2OS