PathHunter® Human TSLP Signaling Reporter Cell-Based Assay Kit (REH)

Background Information:

The PathHunter® Human TSLP Signaling Reporter Cell-Based Assay Kit (REH) utilizes the REH CRLF2 Signaling Pathway Reporter cell line. This engineered cell line monitors the activation of specific signaling pathways, utilizing the established PathHunter Enzyme Fragment Complementation (EFC) technology. It can be used for studying drug candidates (small molecules or biologics) affecting the TSLP pathway. This cell line enables assessment of TSLP pathway stimulation based on changes in the expression of a STAT5 response element-driven reporter protein. The included cell line endogenously expresses IL-7Ra, overexpresses CRLF2, and also expresses PL-tagged reporter protein under the control of a STAT5-inducible transcriptional response element. Pathway activation induces expression of the PL-tagged protein, allowing detection of the EFC signal upon addition of the EA-containing detection reagent.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Cytokine Receptor

Accession Number:

NM_022148

Target Species:

Human

Cell Background:

REH