PathHunter® TRHR Total GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® TRHR Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. TRH) based internalization of TRHR via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged TRHR and a PK-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the TRHR receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Thyrotropin-Releasing Hormone

Sub Family:

Class A

Coupling:

Gq

Protein Name:

TRH

Protein Aliases:

Thyroliberin receptor

Accession Number:

AY493373

UniProt Number:

P34981

Gene Name:

TRHR

Gene ID:

7201

Target Species:

Human

Cell Background:

U2OS