PathHunter® Human TBXA2R Stable Cell Line Activated GPCR Internalization Assay (U2OS)

Background Information:

The PathHunter Human TBXA2R Stable Cell Line Activated GPCR Internalization Assay (U2OS) utilizes the TBXA2R Activated GPCR Internalization cell line. The stable engineered cell line is designed for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. thromboxane A2) based internalization of TBXA2R via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged TBXA2R. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting TBXA2R-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

GPCR

Family:

Prostanoid

Sub Family:

Class A

Coupling:

Gq

Protein Name:

TP (TXA2)

Protein Aliases:

Prostanoid TP receptor

Accession Number:

NM_001060

UniProt Number:

P21731

Gene Name:

TBXA2R

Gene ID:

6915

Target Species:

Human

Cell Background:

U2OS