PathHunter® Human SSTR2 Activated GPCR Internalization Cell-Based Assay Kit (CHO-K1)

Background Information:

The PathHunter® Human SSTR2 Activated GPCR Internalization Cell-Based Assay Kit (CHO-K1) utilizes the SSTR2 Activated GPCR internalization cell line. The stable engineered cell line is designed for use in studying drug candidates (small molecules or biologics) that affect GPCR activation and internalization. This cell line enables the assessment of ligand (e.g., somatostatin 28)- based internalization of SSTR2 via the detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged SSTR2. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting SSTR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Somatostatin

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

sst2

Protein Aliases:

SS2-R|SS2R|SRIF-1

Accession Number:

AY236542

UniProt Number:

P30874

Gene Name:

SSTR2

Gene ID:

6752

Target Species:

Human

Cell Background:

CHO-K1