PathHunter® PROKR2 Total GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® PROKR2 Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. prokinectin, EG-VEGF) based internalization of PROKR2 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses PK-tagged PROKR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PROKR2 receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Prokineticin

Sub Family:

Class A

Coupling:

Gs

Protein Name:

PK2

Protein Aliases:

G-protein coupled receptor 73-like 1|G-protein coupled receptor I5E

Accession Number:

NM_144773

UniProt Number:

Q8NFJ6

Gene Name:

PROKR2

Gene ID:

128674

Gene Aliases:

GPR73b|PKR2|GPRg2|dJ680N4.3|GPR73L1

Target Species:

Human

Cell Background:

U2OS