PathHunter® Human OPRK1 (Opioid Kappa) Total GPCR Internalization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human OPRK1 (Opioid Kappa) Total GPCR Internalization Cell-Based Assay Kit (U2OS) utilizes the OPRK1 Total GPCR internalization cell line. The stable engineered cell line is for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g., dynorphin A) based internalization of OPRK1 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged OPRK1 and a PK-tagged early endosome protein. Upon ligand-induced receptor activation, the resulting internalization of the OPRK1 receptor to the early endosome leads to production of the EFC signal. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Opioid

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

kappa (KOP)

Protein Aliases:

KOR-1

Accession Number:

AF498922

UniProt Number:

P41145

Gene Name:

OPRK1

Gene ID:

4986

Gene Aliases:

KOR|OPRK

Target Species:

Human

Cell Background:

U2OS