PathHunter® NMU1R Total GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® NMU1R Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. neuromedin U-25) based internalization of NMU1R via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses PK-tagged NMU1R and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the NMU1R receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Neuromedin U

Sub Family:

Class A

Coupling:

Gq

Protein Name:

NMU1

Protein Aliases:

G-protein coupled receptor 66|G-protein coupled receptor FM-3

Accession Number:

NM_006056

UniProt Number:

Q9HB89

Gene Name:

NMUR1

Gene ID:

10316

Gene Aliases:

GPC-R|FM-3|NMU1R

Target Species:

Human

Cell Background:

U2OS