PathHunter® MC3R Total GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® MC3R Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. melanocyte stimulating hormone) based internalization of MC3R via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses PK-tagged MC3R and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the MC3R receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Melanocortin

Sub Family:

Class A

Coupling:

Gs

Protein Name:

MC3

Accession Number:

AY227893

UniProt Number:

P41968

Gene Name:

MC3R

Gene ID:

4159

Gene Aliases:

MC3

Target Species:

Human

Cell Background:

U2OS