PathHunter® Human LPS Signaling Reporter Cell-Based Assay Kit (THP-1)

Background Information:

The PathHunter® Human LPS Signaling Reporter Cell-Based Assay Kit (THP-1) utilizes the THP-1 LPS Signaling Pathway Reporter cell line. This engineered cell line monitors the activation of specific signaling pathways, utilizing the established PathHunter Enzyme Fragment Complementation (EFC) technology. It can be used for studying drug candidates (small molecules or biologics) affecting the LPS/endotoxin response pathway. This cell line enables assessment of LPS response pathway stimulation based on changes in the expression of a NF-kB response element-driven reporter protein. The included cell line natively expresses TLR4 complex LPS receptor proteins, and also expresses PL-tagged reporter protein under the control of a NF-kB-inducible transcriptional response element. Pathway activation induces expression of the PL-tagged protein, allowing detection of the EFC signal upon addition of the EA-containing detection reagent.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Pathways

Target Species:

Human

Cell Background:

THP-1