PathHunter® Human IGF1R Functional Kinase Cell-Based Assay Kit (HEK 293)

Background Information:

The PathHunter® Human IGF1R Functional Kinase Cell-Based Assay Kit (HEK 293) utilizes the HEK 293 IGF1R Functional Assay Cell Line. This cell based assay is developed to study drug candidates (small molecules or biologics) targeting receptor tyrosine kinases (RTKs). This kit assesses RTK activation via the detection of IGF1R activation-induced SH2 domain protein recruitment. The included cell line overexpresses PK-tagged Human IGF1R and an EA-tagged SH2 domain protein. Upon ligand (e.g. IGF-1)-induced receptor activation, SH2-EA recruitment leads to the production of an EFC signal proportional to the degree of receptor activation. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

IGF1R

Protein Aliases:

Insulin-like growth factor I receptor|IGF-I receptor|CD antigen CD221 [Cleaved into: Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain]

Accession Number:

NM_000875

UniProt Number:

P08069

Gene Name:

IGF1R

Gene ID:

3480

Gene Aliases:

JTK13|CD221|IGFIR|MGC18216|IGFR

Target Species:

Human

Cell Background:

HEK293