PathHunter® Human GPR152 β-Arrestin Cell-Based Assay Kit (HEK 293)

Background Information:

The PathHunter® Human GPR152 β-Arrestin Cell-Based Assay Kit (HEK 293) utilizes the Human GPR152 β-Arrestin orphan cell line. This engineered stable cell line is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of β-arrestin recruitment following receptor activation for GPCR drug discovery and development programs. This cell line enables the assessment of ligand based activation of GPR152 GPCR activity via detection of β-arrestin recruitment. This cell line employs Enzyme Fragment Complementation (EFC) technology that combines an overexpressed PK-tagged GPR152 target receptor and an EA-tagged β-Arrestin 2 to generate a chemiluminescent signal proportional to receptor activation. Please note that this cell line's target receptor is considered an orphan, and orphan receptors typically do not have established control ligands.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Class A Orphan

Sub Family:

Orphan and other 7TM

Protein Name:

GPR152

Accession Number:

NM_206997.1

UniProt Number:

Q8TDT2

Gene Name:

GPR152

Gene ID:

390212

Gene Aliases:

PGR5

Target Species:

Human

Cell Background:

HEK293