Biological Information
Background Information:
The PathHunter® ADRA1D cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. epinephrine and norepinephrine) based activation of ADRA1D GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged ADRA1D and EA-tagged β-Arrestin 2. Activation of ADRA1D stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Adrenoceptor
Sub Family:
Class A
Coupling:
Gq
Protein Name:
alpha1D
Protein Aliases:
Alpha-1D adrenoreceptor|Alpha-1D adrenoceptor|Alpha-adrenergic receptor 1a
Accession Number:
NM_000678
UniProt Number:
P25100
Gene Name:
ADRA1D
Gene ID:
146
Gene Aliases:
ADRA1R|ADRA1A|ADRA1
Target Species:
Human
Cell Background:
HEK293
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
β-Arrestin Isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®