PathHunter® Human FPRL1 Activated GPCR Internalization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human FPRL1 Activated GPCR Internalization Cell-Based Assay Kit (U2OS) utilizes the FPRL1 Activated GPCR internalization cell line. The stable engineered cell line is designed for use in studying drug candidates (small molecules or biologics) that affect GPCR activation and internalization. This cell line enables the assessment of ligand (e.g., formylpeptides)- based internalization of FPRL1 via the detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged FPRL1. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting FPRL1-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

GPCR

Family:

Formylpeptide

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

FPR2

Protein Aliases:

FMLP-R-I|Formyl peptide receptor-like 1|HM63|Lipoxin A4 receptor|LXA4 receptor|RFP

Accession Number:

AY225226

UniProt Number:

P25090

Gene Name:

FPR2

Gene ID:

2358

Gene Aliases:

LXA4R|HM63|FPRH2|FMLPX|FPR2A|FMLP-R-II|ALXR|FPR|FMLP

Target Species:

Human

Cell Background:

U2OS