PathHunter® FPR1 Total GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® FPR1 Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. formylpeptides) based internalization of FPR1 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses PK-tagged FPR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the FPR1 receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Formylpeptide

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

FPR1

Protein Aliases:

N-formyl peptide receptor|FPR|N-formylpeptide chemoattractant receptor

Accession Number:

NM_002029

UniProt Number:

P21462

Gene Name:

FPR1

Gene ID:

2357

Gene Aliases:

FPR|FMLP

Target Species:

Human

Cell Background:

U2OS