PathHunter® EDNRB Total GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® EDNRB Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. endothelin-3) based internalization of EDNRB via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged EDNRB and a PK-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the EDNRB receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Endothelin

Sub Family:

Class A

Coupling:

Gs & Gi/Go

Protein Name:

ETB

Protein Aliases:

ET-BR|Endothelin receptor non-selective type

Accession Number:

NM_000115.2

UniProt Number:

P24530

Gene Name:

EDNRB

Gene ID:

1910

Gene Aliases:

ETB

Target Species:

Human

Cell Background:

U2OS