PathHunter® Human LPA1 (EDG2) Activated GPCR Internalization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human LPA1 (EDG2) Activated GPCR Internalization Cell-Based Assay Kit (U2OS) utilizes the EDG2 Activated GPCR internalization cell line. The stable engineered cell line is designed for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. LPA) based internalization of EDG2 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged EDG2. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting EDG2-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Lysophospholipid (LPA)

Sub Family:

Class A

Coupling:

Gi/Go & Gq

Protein Name:

LPA1

Protein Aliases:

LPA-1|EDG2|Lysophosphatidic acid receptor Edg-2

Accession Number:

NM_001401.3

UniProt Number:

Q92633

Gene Name:

LPAR1

Gene ID:

1902

Gene Aliases:

edg-2|rec.1.3|vzg-1|Gpcr26|Mrec1.3|LPA1|GPR26

Target Species:

Human

Cell Background:

U2OS