Biological Information
Background Information:
The PathHunter® HTR6 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin/5-HT) based activation of HTR6 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged HTR6 and EA-tagged β-Arrestin 2. Activation of HTR6 stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Serotonin
Sub Family:
Class A
Coupling:
Gq & Gs
Protein Name:
5-HT6
Protein Aliases:
5-HT6|Serotonin receptor 6
Accession Number:
AY429105
UniProt Number:
P50406
Gene Name:
HTR6
Gene ID:
3362
Gene Aliases:
5-HT6|5-HT6R
Target Species:
Human
Cell Background:
DLD1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
β-Arrestin Isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®