PathHunter® Human IL-8 (CXCR1) Activated GPCR Internalization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human IL-8 (CXCR1) Activated GPCR Internalization Cell-Based Assay Kit (U2OS) utilizes the CXCR1 (IL8RA) Activated GPCR internalization cell line. The stable engineered cell line is designed for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables the assessment of ligand (e.g., CXCL8/IL-8)- based internalization of CXCR1 via the detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged CXCR1 (IL8RA). Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting CXCR1 (IL8RA)-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

CXCR1 (IL-8RA)

Protein Aliases:

CXCR-1|CDw128a|High affinity interleukin-8 receptor A|IL-8R A|IL-8 receptor type 1|CD antigen CD181

Accession Number:

NM_000634

UniProt Number:

P25024

Gene Name:

CXCR1

Gene ID:

3577

Gene Aliases:

CKR-1|CDw128a|CD181

Target Species:

Human

Cell Background:

U2OS