PathHunter® Human THR beta Nuclear Receptor Protein Interaction Cell-Based Assay Kit (CHO-K1)

Background Information:

The PathHunter® Human THR beta Nuclear Receptor Protein Interaction Cell-Based Assay Kit (CHO-K1) utilizes the THRβ Protein Interaction cell line. The stable engineered cell line is designed for use in studying drug candidates (small molecules or biologics) affecting NHR activation and recruitment of cofactors. This cell line enables assessment of ligand (e.g., triiodothyronine) based NHR activation via detection of SRCP (steroid receptor co-activator peptide) recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged THRβ NHR and EA-tagged steroid receptor co-activator peptide (SRCP). Upon activation of THRβ, induced interaction with SRCP leads to production of the EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

NHR

Family:

Thyroid Hormone

Sub Family:

Non-Steroid

Protein Name:

TRbeta

Protein Aliases:

avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 2|oncogene ERBA2|generalized resistance to thyroid hormone|thyroid hormone receptor beta 1

Accession Number:

NM_000461

UniProt Number:

P10828

Gene Name:

THRB

Gene ID:

7068

Gene Aliases:

THRB1|THRB2|NR1A2|THR1|ERBA-BETA|GRTH|ERBA2

Target Species:

Human

Cell Background:

CHO-K1