Biological Information

Background Information:

The PathHunter® RXFP4 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. relaxin-3) based activation of RXFP4 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged RXFP4 and EA-tagged β-Arrestin 2. Activation of RXFP4 stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Relaxin Family Peptide

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

RXFP4

Protein Aliases:

G-protein coupled receptor 100|G-protein coupled receptor GPCR142|Insulin-like peptide INSL5 receptor|Relaxin family peptide receptor 4

Accession Number:

NM_181885.2

UniProt Number:

Q8TDU9

Gene Name:

RXFP4

Gene ID:

339403

Gene Aliases:

GPR100|RLN3R2

Target Species:

Human

Cell Background:

CHO-K1

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

β-Arrestin Isoform:

β-Arrestin-2

Assay Measures:

β-Arrestin Recruitment

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®