PathHunter® Human MR Nuclear Receptor Protein Interaction Cell-Based Assay Kit (CHO-K1)

Background Information:

The PathHunter® Human MR Nuclear Receptor Protein Interaction Cell-Based Assay Kit (CHO-K1) utilizes the MR Protein Interaction cell line. The stable engineered cell line is designed for use in studying drug candidates (small molecules or biologics) affecting NHR activation and recruitment of cofactors. This cell line enables assessment of ligand (e.g. aldosterone) based NHR activation via detection of SRCP (steroid receptor co-activator peptide) recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged MR NHR and EA-tagged steroid receptor co-activator peptide (SRCP). Upon activation of MR, induced interaction with SRCP leads to production of the EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

NHR

Family:

3-Ketosteroid

Sub Family:

Steroid

Protein Name:

MCR

Protein Aliases:

Nuclear receptor subfamily 3 group C member 2

Accession Number:

NM_000901

UniProt Number:

P08235

Gene Name:

NR3C2

Gene ID:

4306

Gene Aliases:

MR|MCR|MLR

Target Species:

Human

Cell Background:

CHO-K1