PathHunter® Human CRTH2 β-Arrestin Cell-Based Assay Kit (CHO-K1)

Background Information:

The PathHunter® Human CRTH2 β-Arrestin Cell-Based Assay Kit (CHO-K1) utilizes the Human CRTH2 β-Arrestin cell line. This engineered stable cell line is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of β-arrestin recruitment following receptor activation for GPCR drug discovery and development programs. This cell line enables the assessment of ligand (e.g., prostaglandin D2) based activation of CRTH2 GPCR activity via detection of β-arrestin recruitment. This cell line employs Enzyme Fragment Complementation (EFC) technology that combines an overexpressed PK-tagged CRTH2 target receptor and an EA-tagged β-Arrestin 2 to generate a chemiluminescent signal proportional to receptor activation. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Prostanoid

Sub Family:

Class A

Protein Name:

DP2

Protein Aliases:

chemoattractant receptor homologous molecule expressed on T helper type 2 cells|G-protein coupled receptor 44|CD antigen CD294

Accession Number:

AY507142

UniProt Number:

Q9Y5Y4

Gene Name:

PTGDR2

Gene ID:

11251

Gene Aliases:

CRTH2|CD294|DP2|DL1R|GPR44

Target Species:

Human

Cell Background:

CHO-K1