Biological Information
Background Information:
The PathHunter® CCRL2 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CCL19) based activation of CCRL2 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged CCRL2 and EA-tagged β-Arrestin 2. Activation of CCRL2 stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Chemokine
Sub Family:
Class A
Protein Name:
CCRL2
Protein Aliases:
Chemokine receptor X|Putative MCP-1 chemokine receptor
Accession Number:
NM_003965
UniProt Number:
O00421
Gene Name:
CCRL2
Gene ID:
9034
Gene Aliases:
HCR|CRAM-B|CKRX|CRAM-A|ACKR5
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
β-Arrestin Isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®