Biological Information
Background Information:
The PathHunter® CCR7 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CCL19/MIP-3β) based activation of CCR7 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged CCR7 and EA-tagged β-Arrestin 2. Activation of CCR7 stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Chemokine
Sub Family:
Class A
Coupling:
Gi/Go
Protein Name:
CCR7
Protein Aliases:
CC-CKR-7|CCR-7|BLR2|CDw197|Epstein-Barr virus-induced G-protein coupled receptor 1|EBI1|EBV-induced G-protein coupled receptor 1|MIP-3 beta receptor|CD antigen CD197
Accession Number:
AY587876
UniProt Number:
P32248
Gene Name:
CCR7
Gene ID:
1236
Gene Aliases:
BLR2|CDw197|CD197
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
β-Arrestin Isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®