Biological Information
Background Information:
The PathHunter® CCR6 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CCL20/MIP-3α) based activation of CCR6 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged CCR6 and EA-tagged β-Arrestin 2. Activation of CCR6 stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Chemokine
Sub Family:
Class A
Coupling:
Gi/Go
Protein Name:
CCR6
Protein Aliases:
CC-CKR-6|CCR-6|Chemokine receptor-like 3|CKR-L3|DRY6|G-protein coupled receptor 29|GPR-CY4|GPRCY4|LARC receptor|CD antigen CD196
Accession Number:
AY242126
UniProt Number:
P51684
Gene Name:
CCR6
Gene ID:
1235
Gene Aliases:
CKR-L3|GPR-CY4|CMKBR6|GPR29|DRY-6|DCR2|BN-1|CD196
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
β-Arrestin Isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®