PathHunter® Human MDC (CCR4) Total GPCR Internalization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human MDC (CCR4) Total GPCR Internalization Cell-Based Assay Kit (U2OS) utilizes the CCR4 Total GPCR internalization cell line. The stable engineered cell line is for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g., CCL22/MDC) based internalization of CCR4 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses PK-tagged CCR4 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, the internalization of the CCR4 receptor to the early endosome results in the production of the EFC signal. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

CCR4

Protein Aliases:

CC-CKR-4|CCR-4|CCR4|K5-5|CD antigen CD194

Accession Number:

AY322539

UniProt Number:

P51679

Gene Name:

CCR4

Gene ID:

1233

Gene Aliases:

CC-CKR-4|CMKBR4|CKR4|k5-5|ChemR13|CD194

Target Species:

Human

Cell Background:

U2OS