PathHunter® AVPR1B Activated GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® AVPR1B Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. vasopressin) based internalization of AVPR1B via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged AVPR1B. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting AVPR1B-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Vasopressin and Oxytocin

Sub Family:

Class A

Coupling:

Gq

Protein Name:

V1B

Protein Aliases:

AVPR V1b|AVPR V3|Antidiuretic hormone receptor 1b|Vasopressin V3 receptor

Accession Number:

NM_000707.3

UniProt Number:

P47901

Gene Name:

AVPR1B

Gene ID:

553

Gene Aliases:

AVPR3|VPR3

Target Species:

Human

Cell Background:

U2OS