PathHunter® ADRA2C Total GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® ADRA2C Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. epinephrine/norepinephrine) based internalization of ADRA2C via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged ADRA2C and a PK-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the ADRA2C receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Adrenoceptor

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

alpha2C

Protein Aliases:

Alpha-2C adrenoreceptor|Alpha-2C adrenoceptor|Alpha-2CAR

Accession Number:

NM_000683

UniProt Number:

P18825

Gene Name:

ADRA2C

Gene ID:

152

Gene Aliases:

ADRA2L2|ADRA2RL2

Target Species:

Human

Cell Background:

U2OS