PathHunter® Human ADRA2C Total GPCR Internalization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human ADRA2C Total GPCR Internalization Cell-Based Assay Kit (U2OS) utilizes the ADRA2C Total GPCR internalization cell line. The stable engineered cell line is for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. epinephrine/norepinephrine) based internalization of ADRA2C via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged ADRA2C and a PK-tagged early endosome protein. Upon ligand-induced receptor activation, the internalization of the ADRA2C receptor to the early endosome results in the production of the EFC signal. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Adrenoceptor

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

alpha2C

Protein Aliases:

Alpha-2C adrenoreceptor|Alpha-2C adrenoceptor|Alpha-2CAR

Accession Number:

NM_000683

UniProt Number:

P18825

Gene Name:

ADRA2C

Gene ID:

152

Gene Aliases:

ADRA2L2|ADRA2RL2

Target Species:

Human

Cell Background:

U2OS