InCELL Hunter™ Human FGR Kinase Target Engagement Cell-Based Assay Kit (HEK 293)

Background Information:

The InCELL Hunter™ Human FGR Kinase Target Engagement Cell-Based Assay Kit (HEK 293) utilizes the InCELL Hunter™ HEK 293 FGR Kinase Binding cell line. The stable engineered cell line is intended for use in studying small molecule drug candidates that engage with kinases. This cell line enables assessment of compound-engagement-based stabilization of FGR via detection of FGR levels. This product uses EFC technology. The cell line included overexpresses FGR's kinase domain fused to PL. In the absence of a compound that binds and stabilizes the target, this protein fragment is naturally degraded and no EFC signal is produced. When the compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Kinase

Family:

TK

Sub Family:

Protein Tyrosine

Protein Name:

FGR

Protein Aliases:

Gardner-Rasheed feline sarcoma viral|v-fgr oncogene homolog|Proto-oncogene c-Fgr|p55-Fgr|p58-Fgr|p58c-Fgr

Accession Number:

NM_000163

UniProt Number:

P09769

Gene Name:

FGR

Gene ID:

2268

Gene Aliases:

c-fgr|p55c-fgr

Target Species:

Human

Cell Background:

HEK293