InCELL Hunter™ Human BRDT(1,2) Bromodomain Target Engagement Cell-Based Assay Kit (HEK 293)

Background Information:

The InCELL Hunter™ Human BRDT(1,2) Bromodomain Target Engagement Cell-Based Assay Kit (HEK 293) utilizes the InCELL Hunter™ HEK 293 BRDT(1,2) Bromodomain cell line. The stable engineered cell line is intended for use in studying small molecule drug candidates that engage with regulators of epigenetics pathways. This cell line enables assessment of compound-engagement-based stabilization of BRDT(1,2) via detection of BRDT(1,2) levels. This product uses EFC technology. The cell line included overexpresses BRDT's first and second bromodomains fused to PL. In the absence of a compound that binds and stabilizes the target, this protein fragment is naturally degraded, and no EFC signal is produced. When the compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Epigenetic

Family:

Bromodomain-Containing

Sub Family:

Reader

Protein Name:

BRDT

Protein Aliases:

cancer/testis antigen 9

Accession Number:

NM_001726

UniProt Number:

Q58F21

Gene Name:

BRDT

Gene ID:

676

Gene Aliases:

BRD6|CT9

Target Species:

Human

Cell Background:

HEK293