InCELL Hunter™ Human BRD3(1,2) Bromodomain Target Engagement Cell-Based Assay Kit (HEK 293)

Background Information:

The InCELL Hunter™ Human BRD3(1,2) Bromodomain Target Engagement Cell-Based Assay Kit (HEK 293) utilizes the InCELL Hunter™ HEK 293 BRD3(1,2) Bromodomain cell line. The stable engineered cell line is intended for use in studying small molecule drug candidates that engage with regulators of epigenetic pathways. This cell line enables assessment of compound-engagement-based stabilization of BRD3(1,2) via detection of BRD3(1,2) levels. This product uses EFC technology. The cell line included overexpresses BRD3's first and second bromodomains fused to PL. In the absence of a compound that binds and stabilizes the target, this protein fragment is naturally degraded, and no EFC signal is produced. When the compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Epigenetic

Family:

Bromodomain-Containing

Sub Family:

Reader

Protein Name:

BRD3

Protein Aliases:

RING3-like

Accession Number:

NM_007371.3

UniProt Number:

Q15059

Gene Name:

BRD3

Gene ID:

8019

Gene Aliases:

RING3L|ORFX|KIAA0043

Target Species:

Human

Cell Background:

HEK293