cAMP Hunter™ Human GPR109A Gi Cell-Based Assay Kit (CHO-K1)

Background Information:

The cAMP Hunter™ Human GPR109A Gi Cell-Based Assay Kit (CHO-K1) utilizes the Human GPR109A cAMP cell line. This engineered stable cell line is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of cAMP signaling following receptor activation for drug discovery and development programs. This cell line enables assessment of ligand (e.g. nicotinic acid) based activation of GPR109A GPCR activity via detection of decreasing levels of intracellular cAMP. This assay utilizes EFC technology to measure ligand-induced activation by quantifying intracellular cAMP levels. The cells overexpress wild type GPR109A, a naturally Gi/Go coupled GPCR. Upon activation of GPR109A, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This product leverages a sensitive chemiluminescent cAMP detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

GPCR

Family:

Hydroxycarboxylic Acid

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

GPR109A

Protein Aliases:

niacin receptor 1|HCA2

Accession Number:

NM_177551

UniProt Number:

Q8TDS4

Gene Name:

HCAR2

Gene ID:

338442

Gene Aliases:

HCA2|HM74A|PUMAG|Puma-g|NIACR1

Target Species:

Human

Cell Background:

CHO-K1