cAMP Hunter™ Rat DRD2S Cell-Based Assay Kit (CHO-K1)

Background Information:

The cAMP Hunter™ Rat DRD2S Cell-Based Assay Kit (CHO-K1) utilizes the Rat DRD2S cAMP cell line. This engineered stable cell line is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of cAMP signaling following receptor activation for drug discovery and development programs. This cell line enables assessment of ligand (e.g. dopamine, pergolide) based activation of rDRD2S GPCR activity via detection of decreasing levels of intracellular cAMP. This assay utilizes EFC technology to measure ligand-induced activation by quantifying intracellular cAMP levels. The cells overexpress wild type rDRD2S, a naturally Gi/Go coupled GPCR. Upon activation of rDRD2S, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This product leverages a sensitive chemiluminescent cAMP detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Dopamine

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

D2

Accession Number:

M36831

UniProt Number:

P61169

Gene Name:

DRD2

Gene ID:

24318

Target Species:

Rat

Cell Background:

CHO-K1