cAMP Hunter™ CHO-K1 HTR5A Gi Cell Line

Background Information:

The cAMP Hunter™ HTR5A Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin/5-HT) based activation of HTR5A GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type HTR5A , a naturally Gi/Go coupled GPCR. Upon activation of HTR5A , the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Serotonin

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

5-HT5A

Protein Aliases:

5-HT-5A|5-HT5A|Serotonin receptor 5A

Accession Number:

NM_024012

UniProt Number:

P47898

Gene Name:

HTR5A

Gene ID:

3361

Gene Aliases:

5-HT5A

Target Species:

Human

Cell Background:

CHO-K1