Biological Information

Background Information:

The cAMP Hunter™ EDG2 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. 1-oleoyl-LPA) based activation of EDG2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type EDG2 , a naturally Gi/Go coupled GPCR. Upon activation of EDG2 , the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Lysophospholipid (LPA)

Sub Family:

Class A

Coupling:

Gi/Go & Gq

Protein Name:

LPA1

Protein Aliases:

LPA-1|EDG2|Lysophosphatidic acid receptor Edg-2

Accession Number:

NM_001401.3

UniProt Number:

Q92633

Gene Name:

LPAR1

Gene ID:

1902

Gene Aliases:

edg-2|rec.1.3|vzg-1|Gpcr26|Mrec1.3|LPA1|GPR26

Target Species:

Human

Cell Background:

CHO-K1

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

Assay Measures:

cAMP Detection

Detection Method:

Chemiluminescence

Additional Information

Brand:

cAMP Hunter™