cAMP Hunter™ CHO-K1 CXCR5 Gi Cell Line

Background Information:

The cAMP Hunter™ CXCR5 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CXCL13/BCA-1/BLC) based activation of CXCR5 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CXCR5 , a naturally Gi/Go coupled GPCR. Upon activation of CXCR5 , the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

CXCR5

Protein Aliases:

CXCR-5|Burkitt lymphoma receptor 1|Monocyte-derived receptor 15|MDR-15|CD antigen CD185

Accession Number:

NM_001716

UniProt Number:

P32302

Gene Name:

CXCR5

Gene ID:

643

Gene Aliases:

MDR15|CD185

Target Species:

Human

Cell Background:

CHO-K1