cAMP Hunter™ CHO-K1 AGTRL1 Gi Cell Line

Background Information:

The cAMP Hunter™ AGTRL1 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. apelin-13) based activation of AGTRL1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type AGTRL1 , a naturally Gi/Go coupled GPCR. Upon activation of AGTRL1 , the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Apelin

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

APJ

Protein Aliases:

APJ (apelin) receptor

Accession Number:

NM_005161

UniProt Number:

P35414

Gene Name:

APLNR

Gene ID:

187

Gene Aliases:

FLJ90771|APJ|APJR

Target Species:

Human

Cell Background:

CHO-K1