cAMP Hunter™ Human ADCYAP1R1 (PAC1) Cell-Based Assay Kit (CHO-K1)

Background Information:

The cAMP Hunter™ Human ADCYAP1R1 (PAC1) Cell-Based Assay Kit (CHO-K1) utilizes the Human ADCYAP1R1 (PAC1) cAMP cell line. This engineered stable cell line is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of cyclic AMP (cAMP) signaling following receptor activation for drug discovery and development programs. This cell line enables assessment of ligand (e.g. PACAP) based activation of ADCYAP1R1 GPCR activity via detection of increasing levels of intracellular cAMP. This assay utilizes EFC technology to measure ligand-induced activation by quantifying intracellular cAMP levels. The cells overexpress wild type ADCYAP1R1, a naturally Gs coupled GPCR. Upon activation of ADCYAP1R1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This product leverages a sensitive chemiluminescent cAMP detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

VIP and PACAP

Sub Family:

Class B

Coupling:

Gq & Gs

Protein Name:

PAC1 (PACAP)

Protein Aliases:

PACAP-R-1|PACAP-R1|PACAP receptor 1

Accession Number:

AY366498; NM_001118

UniProt Number:

P41586

Gene Name:

ADCYAP1R1

Gene ID:

117

Gene Aliases:

PAC1|PACAPR|PAC1R

Target Species:

Human

Cell Background:

CHO-K1