Ready-to-Assay PTH1 Parathyroid Hormone Receptor Frozen Cells

Background Information:

The Ready-to-Assay PTH1 Parathyroid Hormone Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. parathyroid hormone) based activation of PTH1R GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress PTH1R alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PTH1R , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Parathyroid Hormone

Sub Family:

Class B

Protein Name:

PTH1

Protein Aliases:

PTH/PTHr receptor|Parathyroid hormone 1 receptor|PTH1 receptor

Accession Number:

NM_000316

UniProt Number:

Q03431

Gene Name:

PTH1R

Gene ID:

5745

Target Species:

Human

Cell Background:

Chem-1