Ready-to-Assay PAR2 Receptor Frozen Cells

Background Information:

The Ready-to-Assay PAR2 Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. PAR2) based activation of F2RL1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress F2RL1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of F2RL1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Proteinase-Activated

Sub Family:

Class A

Protein Name:

PAR2

Protein Aliases:

proteinase-activated receptor-2

Accession Number:

NM_005242.4

UniProt Number:

P55085

Gene Name:

F2RL1

Gene ID:

2150

Gene Aliases:

PAR2|GPR11

Target Species:

Human

Cell Background:

Chem-1