Ready-to-Assay CCR5 Chemokine Receptor Frozen Cells

Background Information:

The Ready-to-Assay CCR5 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. MIP, RANTES) based activation of CCR5 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress CCR5 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CCR5 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Protein Name:

CCR5

Protein Aliases:

CC-CKR-5|CCR-5|CCR5|CHEMR13|HIV-1 fusion coreceptor|CD antigen CD195

Accession Number:

NM_001042773.2

UniProt Number:

P51681

Gene Name:

CCR5

Gene ID:

1234

Gene Aliases:

CKR-5|CC-CKR-5|CKR5|CD195|IDDM22

Target Species:

Human

Cell Background:

Chem-1