Ready-to-Assay B2 Bradykinin Receptor Frozen Cells

Background Information:

The Ready-to-Assay B2 Bradykinin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. bradykinin) based activation of BDKRB2 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress BDKRB2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of BDKRB2 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Bradykinin

Sub Family:

Class A

Protein Name:

B2

Protein Aliases:

BK-2 receptor

Accession Number:

M88714

UniProt Number:

P30411

Gene Name:

BDKRB2

Gene ID:

624

Gene Aliases:

BK-2|BKR2

Target Species:

Human

Cell Background:

Chem-1