Biological Information
Background Information:
The ADORA2A Gq cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. adenosine, NECA) based activation of ADORA2A GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADORA2A , a naturally Gq coupled GPCR. Upon activation of ADORA2A , the resulting calcium activity can be fluorescently assayed using the Calcium NoWash PLUS Detection Kit.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Adenosine
Sub Family:
Class A
Coupling:
Gq & Gs
Protein Name:
A2A
Accession Number:
NM_000675.4
UniProt Number:
P29274
Gene Name:
ADORA2A
Gene ID:
135
Gene Aliases:
RDC8|ADORA2
Target Species:
Human
Cell Background:
RBL-2H3
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
Assay Measures:
Calcium Flux
Detection Method:
Chemiluminescence
Additional Information
Brand:
Eurofins DiscoverX®