Biological Information
Background Information:
The ChemiBRITE FFA1/GPR40 Free Fatty Acid Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. free fatty acids) based activation of FFAR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FFAR1 , a naturally Gq coupled GPCR. ChemiBRITE cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of FFAR1 , the resulting calcium activity can be assayed in either detection mode.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Free Fatty Acid
Sub Family:
Class A
Protein Name:
FFA1 (GPR40)
Accession Number:
NM_005303.2
UniProt Number:
O14842
Gene Name:
FFAR1
Gene ID:
2864
Gene Aliases:
FFA1R
Target Species:
Human
Cell Background:
HEK293
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
Assay Measures:
Calcium Flux
Detection Method:
Fluorescence
Additional Information
Brand:
ChemiBRITE