ChemiBRITE A2A Adenosine Receptor Stable Cell Line

Background Information:

The ChemiBRITE A2A Adenosine Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of ADORA2A GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADORA2A alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBRITE cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of ADORA2A , the resulting calcium activity can be assayed in either detection mode.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Adenosine

Sub Family:

Class A

Protein Name:

A2A

Accession Number:

NM_000675.4

UniProt Number:

P29274

Gene Name:

ADORA2A

Gene ID:

135

Gene Aliases:

RDC8|ADORA2

Target Species:

Human

Cell Background:

HEK293